This handbook provides operational guidance for child protection staff and other actors working on prevention and response to family separation in emergencies. It sets out to ensure that responses meet agreed inter-agency standards and are appropriate to the particular situation of individual unaccompanied and separated children, emphasizing the importance of working with affected communities, including children themselves. It accompanies the Toolkit (see Linked Resources section). The handbook is divided into a summary guidance section, which collates all the essential actions and tools covered in the handbook and acts as a quick reference guide for practitioners. Detailed guidance on each topic can be found within corresponding chapters in sections one and Two. Section one provides an overview of the legal framework and organizational mandates related to unaccompanied and separated children (UASC), characterizes family separation in emergencies, describes the causes of separation, outlines how to prepare for and prevent separation in emergencies, and how to undertake coordination and assessment. It also covers the basics of UASC programming, case management and information management. Section two provides detailed guidance on immediate needs in emergencies and detailed information on identification, documentation, alternative care, family tracing, verification, reunification, reintegration and cross-sector coordination.